Template:Table of blood sampling tubes

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Vacutainer/sample tube types for venipuncture/phlebotomy   edit
Tube cap color or type in order of draw Additive Usage and comments
Blood culture bottle Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms Usually drawn first for minimal risk of contamination.[1] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms.[2]
Light blue Sodium citrate (weak calcium chelator/anticoagulant) Coagulation tests such as prothrombin time (PT) and partial thromboplastin time (PTT) and thrombin time (TT). Tube must be filled 100%.
Plain red No additive Serum: Total complement activity, cryoglobulins
Gold (sometimes red and grey "tiger top"[3]) Clot activator and serum separating gel[4] Serum-separating tube: Tube inversions promote clotting. Most chemistry, endocrine and serology tests, including hepatitis and HIV.
Dark green Sodium heparin (anticoagulant) Chromosome testing, HLA typing, ammonia, lactate
Light green Lithium heparin (anticoagulant) Plasma. Tube inversions prevent clotting
Lavender ("purple") EDTA (chelator / anticoagulant) Whole blood: CBC, ESR, Coombs test, platelet antibodies, flow cytometry, blood levels of tacrolimus and cyclosporin
Pink EDTA (chelator / anticoagulant) Blood typing and cross-matching, direct Coombs test, HIV viral load
Royal blue EDTA (chelator / anticoagulant) Trace elements, heavy metals, most drug levels, toxicology
Tan EDTA (chelator / anticoagulant) Lead
Gray Glucose, lactate[6]
Yellow Acid-citrate-dextrose A (anticoagulant) Tissue typing, DNA studies, HIV cultures
Pearl ("white") Separating gel and (K2)EDTA PCR for adenovirus, toxoplasma and HHV-6
  1. ↑ Pagana, KD; Pagana, TJ; Pagana, TN (19 September 2014). Mosby's Diagnostic and Laboratory Test Reference - E-Book. Elsevier Health Sciences. p. xiii. ISBN 978-0-323-22592-2.
  2. ↑ "Chapter 3.4.1: Blood cultures; general detection and interpretation". Clinical Microbiology Procedures Handbook. Wiley. 6 August 2020. ISBN 978-1-55581-881-4.
  3. ↑ "Test Tube Guide and Order of Draw" (PDF). Guthrie Laboratory Services. June 2019.
  4. ↑ "Specimen requirements/containers". Pathology & Laboratory Medicine, UCI School of Medicine. Retrieved 2020-09-10.
  5. ↑ Castellini MA, Castellini JM, Kirby VL (1992). "Effects of standard anticoagulants and storage procedures on plasma glucose values in seals". J Am Vet Med Assoc. 201 (1): 145–8. PMID 1644639.
  6. ↑ Amitava Dasgupta; Jorge L. Sepulveda (20 July 2019). Accurate Results in the Clinical Laboratory: A Guide to Error Detection and Correction. Elsevier Science. p. 131. ISBN 978-0-12-813777-2.
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